Alcohol formula

Formulation and testing of alcohol-based hand sanitizers

This article was originally published here

FASEB J. 2022 May;36 Suppl 1. doi: 10.1096/fasebj.2022.36.S1.R5727.


The pandemic caused as a result of Covid-19 has raised awareness about the use of hand sanitizers around the world. It has been confirmed that maintaining hand hygiene is essential to reduce the colonization and incidence of infectious diseases. The objective of this study was to create an alcohol-based hand sanitizer formulation effective in reducing the presence of microorganisms. A comparative evaluation of the antimicrobial efficacy of different hand sanitizers was used to compare two leading brand hand sanitizers to the 2 formulations manufactured in the experiment. A modified Kirby-Bauer zone of inhibition method was used to monitor the reduction of live cells of E. coli in the presence of each hand sanitizer. Formulation 1 was found to be the most effective, having shown the highest zone of inhibition during the study. Formulation 1 consisted of: 75% isopropyl alcohol, 1.45% glycerol, 0.125% hydrogen peroxide and water. The hydrogen peroxide in the formulation has proven to be the missing ingredient when compared to most leading brand hand sanitizers. The formulation will be tested further to find out which microorganisms may not be affected by this solution.

PMID:35556716 | DOI:10.1096/fasebj.2022.36.S1.R5727